WTB information

This iPSC line was derived from a skin biopsy of a 42 year old Hispanic female donor, who was in good health and had a normal EKG at the time of skin biopsy. The original fibroblasts were reprogrammed using episomal methods with the following factors: LIN28A, MYC (L-MYC), POU5F1 (OCT4), and SOX2 (Okita et al Nat Methods 2011, PMID: 21460823, Addgene# 27077, 27078, 27080). The iPSC line was tested for genomic integration of reprogramming vectors using qPCR, but no integration was detected. It also has a normal karyotype (46, XX), as shown by cytogenetic testing using FISH methodologies. Immunostaining and RT-PCR have been used to characterize the undifferentiated iPSC line for the pluripotency state, while its pluripotency and differentiation potential has been characterized via teratoma formation, embryoid formation, and cardiomyocyte and neural crest differentiation.

WTB-public exome


 

WTB RNA-Seq data of iPSC-cardiomyocyte differentiation by Wnt pathway modulation:
11 time-points of the iPSC to CM.


http://genome.ucsc.edu/cgi-bin/hgTracks?hgS_doOtherUser=submit&hgS_otherUserName=alexgraehl&hgS_otherUserSessionName=ms-153-matt-spindler-d0-through-d60-hg19-sep-2013

(The protocol used for cardiomyocyte differentiation is adapted from: Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK, Zhang J, Kamp TJ, Palecek SP. (2012). Proc. Natl. Acad. Sci. U S A. Jul 3;109(27). PMID: 22645348).

Publications:
Isolation of single-base genome-edited human iPS cells without antibiotic selection.  Yuichiro Miyaoka, Amanda H. Chan, Luke M. Judge, Jennie Yoo, Miller Huang, Trieu D. Nguyen, Paweena P. Lizarraga, Po-Lin So, and Bruce R. Conklin. Nat Methods. Mar 2014; 11(3): 291–293. PMCID: PMC4063274
 
A robust method to derive functional neural crest cells from human pluripotent stem cells.  Faith R. Kreitzer, Nathan Salomonis, Alice Sheehan, Miller Huang, Jason S. Park, Matthew J. Spindler, Paweena Lizarraga, William A. Weiss, Po-Lin So, and Bruce R. Conklin.  Am J Stem Cell 2013;2(2):119-131. PMCID:PMC3708511