WTC Information

Coriell Cat. # GM25256

The WTC line is used as a normal control by research groups all over the world. This iPSC line was derived from a skin biopsy from an healthy adult Asian male donor in his early thirties, who showed normal function in a battery of tests including EKG, and head MRI. The original fibroblasts were reprogrammed using episomal methods with the following factors: LIN28A, MYC (L-MYC), POU5F1 (OCT4), and SOX2(Okita el al Nat Methods 2011, PMID: 21460823, Addgene# 27077, 27078, 27080). The iPSC line was tested for genomic integration of reprogramming factors using qPCR, but no integration was detected. It also has a normal karyotype (46, XY), as shown by cytogenetic testing using FISH methodologies.  Immunostaining and RT-PCR have been used to characterize the undifferentiated iPSC line for the pluripotency state, while its pluripotency and differentiation potential has been characterized via teratoma formation, embryoid formation, and cardiomyocyte and neural crest differentiation.  

WTC is the parental line for the Allen Institute Cell Collection. http://www.allencell.org/

STR Analysis of WTC


Primary References:

  • Isolation of single-base genome-edited human iPS cells without antibiotic selection.Yuichiro Miyaoka, Amanda H. Chan, Luke M. Judge, Jennie Yoo, Miller Huang, Trieu D. Nguyen, Paweena P. Lizarraga, Po-Lin So, and Bruce R. Conklin. Nat Methods. Mar 2014; 11(3): 291–293. PMCID: PMC4063274
  • CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs.  Mo A. Mandegar, Nathaniel Huebsch, Ekaterina B. Frolov, Edward Shin, Truong A, Michael P. Olvera, Amanda H. Chan, Yuichiro Miyaoka, Kristen Holmes, Ian C. Spencer, Luke M. Judge, Gordon DE, Tilda V. Eskildsen , Jacqueline E. Villalta, Max A. Horlbeck,  Luke A. Gilbert, Nevan J. Krogan, Soren P. Sheikh, Jonathan S. Weissman, Stanley L. Qi, Po-Lin So, and Bruce R. Conklin. Cell Stem Cell. Apr 2016; 18(4):541-53. PMID: 26971820

Other References:

  • Stem cells: the new "model organism". Drubin David G., Hyman Anthony A. Mol Biol Cell. 2017 June1;28 (11):1409-1411. PMID:28559439. PMCID: PMC5449140
  • A robust method to derive functional neural crest cells from human pluripotent stem cells.  Faith R. Kreitzer, Nathan Salomonis, Alice Sheehan, Miller Huang, Jason S. Park, Matthew J. Spindler, Paweena Lizarraga, William A. Weiss, Po-Lin So, and Bruce R. Conklin.  Am J Stem Cell 2013;2(2):119-131. PMID:23862100, PMCID:PMC3708511
  • Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem-Derived Cardiomyocytes Cultured over Different Spatial Scales.  Nathaniel Huebsch, Peter Loskill, Mohammad A. Mandegar, Natalie C. Marks, Alice S. Sheehan, Zhen Ma, Anurag Mathur, Trieu N. Nguyen, Jennie C. Yoo, Luke M. Judge, C. Ian Spencer, Anand C. Chukka, Caitlin R. Russell, Po-Lin So, Bruce R. Conklin, and Kevin E. Healy.  Tissue Eng Part C Methods. 2014 Oct 21. PMID:25333967
  • Miniaturized iPS-cell-derived cardiac muscles for physiologically relevant drug response analyses. NathanielHuebsch, Peter Loskill, Nikhil Deveshwar, Ian C Spencer, Luke M. Judge, Mo A. Mandegar, Cade B. Fox, Tamer M. Mohamed, Zhen Ma, Alice M. Sheehan, Annie Truong, Michael Saxton, Jennie Yoo, Deepak Srivastava, Tejal A. Desai, Po-Lin So, Kevin E. Healy, Bruce R. Conklin. Sci Rep. Apr 2016; 6:24726. PMID: 27095412

  • A BAG3 Chaperone Complex Maintains Cardiomyocyte Function During Proteotoxic Stress. Luke M. Judge, Juan A. Perez-Bermejo, Annie Truong, Alexandre J. Ribeiro, Jennie C. Yoo, Chrisina L. Jensen, Mohammad A. Mandegar, Nathaniel Huebsch,   Robyn M. Kaake, Po-Lin So, Deepak Srivastava, Beth L. Pruitt, Nevan J. Krogan, Bruce R. Conklin. JCI Instight. 2017 Jul 20;2 (14). PMID:2872793