Bruneau Lab Upright Microscope

Leica DM5000 B with epifluorescence, bright field, DIC, and dark field capabilities.  It has a Leica DFC 500 camera for color imaging.  

System is running Leica Application Suite LAS 3.7 on a 2013 PC workstation.

Training and support available through the core, please contact us for more details.

Sample Preparation:
This is an upright microscope with a wide range of objectives for standard coverslipped slides.    The 63x objective is optimized for #1 or #1.5 coverslips, with a thickness averaging of 0.17mm.

Cubes available: 

Fluorophore Exciter Filter Dichroic Filter Emission Filter links
Texas Red BP 560/40 595 BP 645/75 Leica Tx2
FITC / Alexa 488 BP 480/40 505 BP 527/30 Leica L5
DAPI / Hoescht BP 360/40 400 BP 470/40 Leica A4
Cy5.5 BP 682/12 697 BP 721/42 Leica Cy5.5



Objectives available (including links to objective pages):

1.25x PL Fluotar / 0.04NA
2.5x N Plan / 0.07NA
5x PL Fluotar / 0.15NA / DIC
10x Plan Apo / 0.4NA / DIC

20x Plan Apo / 0.7NA / DIC
40x Plan Apo / 0.85NA / DIC
63x Plan Apo / 1.4NA (Oil) / DIC

Useful Links:

Bruneau upright scope docs - Packet of documents that cover the basic rules of usage and parts of the scope, including the objectives, filter sets, and camera.