How to Use the Core

The Gladstone Transgenic Gene-Targeting Core provides services for investigators at Gladstone, UCSF, and outside institutions.

You must have an approved IACUC protocol for each transgenic/knock-out mouse model.

1. Contact us:

If you have any questions or comments, please send email. We will contact you to answer questions or set up a meeting. We do not design constructs. However, we have access to many investigators and experts that can answer your questions. It’s essential that you make us aware of your plans prior to scheduling service so time is not wasted when you are ready.

2. Request services:

The Gladstone Transgenic Gene Targeting Core is using an online system to streamline the process of ordering, tracking and billing for core service requests. You need to register for an ilab account and request services through ilab.

For anyone outside Gladstone we can not initiate a project without a PO number.

3. Schedule service:

Once we receive the request we can schedule your experiments. The waiting time for microinjection constantly changes depending on demand. Typically it can be scheduled 3-5 weeks from the time we receive the request.

4. Submit samples:

DNA should be submitted and then injection day will be scheduled. Once an ES cell injection request is received an injection day will be scheduled. ES cells can be grown for injection by the investigator, by the Stem Cell Core at Gladstone, or by UCSF ES Cell Targeting Core.

5. Correspondence:

You will be contacted by email when pups are born. The investigator is responsible for collecting tail biopsies. Mice will be transferred after weaning. You must have an IACUC approved protocol and be able to receive/house the mice in your facility. Please make the appropriate arrangements prior to scheduling a project.