Instruments / Services

FACSAriaII by B.D. Biosciences

  • Equipped with 5 lasers (UV, Violet, Blue, Yellow, and Red) and exclusively operated by the Flow Core staff.
    • Up to 17 colors sorting for immunophenotyping
    • Sorting and analysis of CFP, GFP, YFP, mRFP, mTomato, CFP and mCherry gene expression proteins
    • DNA analysis using standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.)
    • Sorting stem cell side populations
    • Sorting into 15ml falcon, 5ml FACS tubes and Eppendorf or single cell sorting into 96-well plates for culture and slides.
    • Biohazardous cell sorting for HIV and HCV infected samples
  • For a detailed table of lasers, filters, and fluorochromes, click here. Requests for sorting appointments are made directly on the iLab Calendar.

LSRII by B.D. Biosciences

  • 13 color flow cytometer equipped with 4 lasers (Blue, Violet, Red, Green) and with High Throughput capabilities (96w and 384 well plates)
  • For LSRII Standard Operating Procedures click here and for a detailed table of lasers, filters, and fluorochromes, click here.

FACSCaliburDxP8 by B.D. Biosciences

  • Easy to use FACS analyzer equipped with 3 lasers (Blue, Yellow/Green, and Red)
  • For Calibur Startup and Shutdown procedures, click here.

MACSQuant VYB by Miltenyi

  • Easy-to-use FACS analyzer equipped with 3 lasers (Blue, Yellow, and Violet) and HTS platform for 96 well plates.
  • For MACSQuantVYB Startup Procedures click here and for a detailed table of lasers, filters, and fluorochromes, click here.

ImageStreamx by Amnis (EMD Millipore)

  • The ImageStreamx from Amnis integrates flow cytometry and imaging (10,000 cells/s and 6 images/cell). 
  • It is equipped with 3 lasers (Blue, Orange, and Violet). Data is collected with the INSPIRE software and can be analyzed with the IDEAS software.
    • Requests for training on the Xstream can be made on the schedule, pending core approval
    • You will receive an email notification once the core has approved your training appointment.
  • For a detailed table of lasers, filters, and fluorochromes, click here.

HTFC by IntelliCyt

  • 4 color flow cytometer equipped with two lasers (Blue and Yellow).
  • Specializes in 96-well and 384-well plates.
  • For a detailed table of lasers, filters, and flourochromes, click here.
    • There are additional filters available upon request.  Please return the HTFC IntelliCyt to the default configuration when done.
    • Additional filters:  515/20 - FITC, Alexa Fluor 488; 660/20 - APC, Alexa Fluor 647; 670/14 - PE-Cy5; 710/50 - PerCP-Cy5.5