Instruments / Services

Attune NXT by Thermo Fisher Scientific

  • Equipped with 4 lasers ( Violet, Blue, Yellow-Green, and Red).
    • 15 parameters
    • Acoustic focusing benchtop analyzer with high throughput sampler.
    • Ideal for Immunophenotyping and signal studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies and microbiological assays.

FACSAriaII by B.D. Biosciences

  • Equipped with 5 lasers (UV, Violet, Blue, Yellow, and Red) and exclusively operated by the Flow Core staff.
    • Up to 17 colors sorting for immunophenotyping
    • Sorting and analysis of CFP, GFP, YFP, mRFP, mTomato, CFP and mCherry gene expression proteins
    • DNA analysis using standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.)
    • Sorting stem cell side populations
    • Sorting into 15ml falcon, 5ml FACS tubes and Eppendorf or single cell sorting into 96-well plates for culture and slides.
    • Biohazardous cell sorting for HIV and HCV infected samples
  • For a detailed table of lasers, filters, and fluorochromes, click here. Requests for sorting appointments are made directly on the iLab Calendar.

LSRII by B.D. Biosciences

  • 13 color flow cytometer equipped with 4 lasers (Blue, Violet, Red, Green) and with High Throughput capabilities (96w and 384 well plates)
  • For LSRII Standard Operating Procedures click here and for a detailed table of lasers, filters, and fluorochromes, click here.

LSR Fortessa X-20 by BD Biosciences

  • Equipped with 5 lasers (UV, Violet, Blue, Yellow-Green, and Red).
    • 20 parameters
    • Detects 18 colors simultaneously
    • HTS can speed through a 96w plate in 15 minutes.

ImageStreamx by Amnis (EMD Millipore)

  • The ImageStreamx from Amnis integrates flow cytometry and imaging (10,000 cells/s and 6 images/cell). 
  • It is equipped with 3 lasers (Blue, Orange, and Violet). Data is collected with the INSPIRE software and can be analyzed with the IDEAS software.
    • Requests for training on the Xstream can be made on the schedule, pending core approval
    • You will receive an email notification once the core has approved your training appointment.
  • For a detailed table of lasers, filters, and fluorochromes, click here.

HTFC by IntelliCyt

  • 4 color flow cytometer equipped with two lasers (Blue and Yellow).
  • Specializes in 96-well and 384-well plates.
  • For a detailed table of lasers, filters, and flourochromes, click here.
    • There are additional filters available upon request.  Please return the HTFC IntelliCyt to the default configuration when done.
    • Additional filters:  515/20 - FITC, Alexa Fluor 488; 660/20 - APC, Alexa Fluor 647; 670/14 - PE-Cy5; 710/50 - PerCP-Cy5.5