GICD Mouse Stem Cell Core Zeiss Axiovert Incubation System

Zeiss Axiovert 200M with epifluorescence, phase, and bright field capabilities.  It has a monochrome camera (Zeiss AxioCam MRm) for optimal fluorescence imaging as well as a color camera (Zeiss AxioCam ICc 3) for bright field applications.  

Fully motorized X, Y and Z allowing mosaics, mark and subsequent find of structures at a later date, plus Z-stacks and deconvolution.

System is running Zen 2012 on a 2013 fully loaded PC workstation.

Training and support available through the core, please contact us for more details.

Sample Preparation:
This is an inverted microscope with incubation chamber capable of imaging standard sized slides and optically suitable plates.  Standard plates can be imaged on the 20x and 40x objectives which have correction collars.

Cubes available: 

Fluorophore Exciter Filter Dichroic Filter Emission Filter links
DsRed BP 550/25 (HE) FT 570 (HE) BP 605/70 (HE) Zeiss 43 HE
FITC / Alexa 488 BP 470/40 (HE) FT 495 (HE) BP 525/50 (HE) Zeiss 38 HE
DAPI / Hoescht BP 365/12 FT 395 LP 397 Zeiss 01
RFP BP 530-585 FT 600 LP 615 Zeiss 00

Objectives available (including links to objective pages):
Plan Neofluar 2.5x / 0.075NA

EC Plan Neofluar 5x / 0.16NA / Phase 
A Plan 10x / 0.25NA / Phase 
Acroplan 20x / 0.4NA / Phase
LD Acroplan 40x / 0.6NA / Phase

Useful Links

Zen 2012 lite - Windows based (no mac) program for opening Zeiss files and converting to other formats, basic manipulation of the images and can provide information on how the original image was taken.

Getting Started docs - Packet of documents that cover the basic rules of usage and parts of the scope, including the objectives and filter sets.

GSCC1 Peripheral docs - Packet of documents that cover the incubator, UV lamp, and cameras.

Zeiss ZEN docs - Quick guides for getting started with ZEN 2012, multidimensional capture, and image export and import, as well as the full user guide.