conklin lab
cardiovascular disease
Information on Human iPS Cells
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NORMAL CONTROL iPSC LINES
- WTC Information
Normal control, male iPSC line - WTB iPSC Line
Normal control, female iPSC line.
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GENETICALLY ENGINEERED VERSIONS OF WTC AND WTB LINES
- WTC GCaMP
The calcium activated version of GFP (GCaMPf6) was targetted to the AAVS1 locus of WTC cells. A strong constitutive promoter (CAG) drives the expression of the GCaMP6f ORF. Puromycin resistant at 0.5 µg/ml (Sensitive to doses greater than 2.0 µg/ml)
Available for Gladstone Stem Cell Core.
- CRISPRi Gen1C
WTC iPSC background, inducible with mCherry and GCaMP6
Mandegar et al 2016, Cell Stem Cell, 18, 541–553
Available from Gladstone Stem Cell Core
- CRISPRi Gen2C
WTC iPSC background, inducible with no fluorophore
Mandegar et al 2016, Cell Stem Cell, 18, 541–553
Available from Gladstone Stem Cell Core
- CRISPRi Gen1B
WTB iPSC background, inducible with mCherry and GCaMP6
Mandegar et al 2016, Cell Stem Cell, 18, 541–553
Available from Gladstone Stem Cell Core
- CRISPRn Gen1C
WTC iPSC background, inducible Cas9 cutting with no fluorophore
Mandegar et al 2016, Cell Stem Cell, 18, 541–553
Available from Gladstone Stem Cell Core
- CRISPRn Gen1B
WTB iPSC background, inducible Cas9 cutting with no fluorophore
Mandegar et al 2016, Cell Stem Cell, 18, 541–553
Available from Gladstone Stem Cell Core
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PATIENT DERIVED iPSC LINES
- LQT2 iPSC Line
Disease line derived from female donor with LQT2 syndrome. - LQT3 iPSC Line
Disease line derived from female donor with LQT3 syndrome.
