finkbeiner lab
center for systems and therapeutics
Protocols
Physical Exam for the Cell
(Download PDF)
Cell Lines
The Cells Grow at 33C
Generation and Characterization of Embryonic Striatal Conditionally Immortalized ST14A Cells
J. Neurosci Res 53:223–234, 1998
ST14A Cells have Properties of a Medium-size Spiny Neuron
Exp. Neurol. 167:231–266, 2001
Cell Culture and Slice Techniques
Brain Slice Culture and Transfection Protocol
Lo Lab - Center for Drug Discovery
Culture of Primary Neurons and Cell Lines
Dissociated Cultures of Cerebellar Neurons
Old protocol from Hank Dudek
Forskolin/IBMX Treatment to Boost Expression
Neuronal culture treatment (for enhanced protein expression)
Poly D-lysine/Laminin Solution
Primary Culture of Cortical Hippocampal Neurons
Most recent protocol for the culture of rat cortical/hippocampal neurons
Primary Striatal Neuron Culture Method
Modified protocol by Jason Miller for improved neuron survival. Method is generalizable to other primary neuron cultures besides striatum. Also contains recipes for all solutions needed. Posted January 2010.
Reagents for Hippcampal Cultures
Greenberg Lab (2/93)
Cytochemistry Techniques
Beta-Gal Staining in Primary Neurons
Z. Xia, Greenberg Lab (4/94)
Immunostaining on Glass Coverslips
Hank Dudek, Greenberg Lab (10/95)
Labeling of Nuclei with BrdU and PI for FACS
Staining Methods for Cell Death
DNA Techniques
Cloning from Beginning to End
Steve's outline of cloning plasmid DNA
CsCl Prep of Plasmid DNA
Finkbeiner
DNA Laddering
Hank Dudek (8/95)
Large-scale DNA preps using DMAE columns
Michael Lin (1998)
Mini-plasmid Preparation
D. Grant McFadden
Preparation of Electrocompetent Bacteria
Michael Lin
Manuals & Spec Sheets
Qiagen QIAquick Spin Handbook
Protocols for PCR purification, nucleotide removal and Gel extraction of DNA
Stragene Quikchange Mutagenesis kit
Macros for Data Analysis in Excel
Analysis of Calcium Imaging Data
Protein Techniques
3B5H10 Fab Purification
Cell Lysis/Western/IP
Complied 6/95 by AJS from AB
Coupling of Phospho-ELKtide to KLH
Human staining with 3B5H10
Nga Bien-Ly
Immunoaffinity Purification of Antibodies
(SEK 12/12/95)
PhosphoCREB Immunostaining (Neurons)
Preparation of Affinity Column
(SEK 5/3/95)
Preparation of Electrocompetent Bacteria
Michael Lin
Protein Buffers
(6/95 by AJS from AB)
Purification of a 6-Liter Prep Expression of TEV Protease, DMH
(May 2006)
Purification of TRX-Exon1Htt39Q-His
Purification of TRXHtt39Q and 3B5H10 Fab Co-complex
Slot Blot Filter Assay Protocol
Membrane filter assay for detection of amyloid-like polyglutamine-containing protein aggregates (book section)
Western Blot for 1C2, 3B5H10, and 4C8 Antibodies
Western Blot for Anti-HA
Method from Marc Diamond
Western Blot for Anti-pCREB
Old protocol from David Ginty
Western Blot of TBP from TBP-GST Bacteria
Cell Lines
Chroma filter orientation instructions
Recipes for Lab Solutions/Buffers
General Stock Solutions For Molecular Biology
Solutions for Primary Neuronal Culture
Complied 6/95 by AJS from AB
RNA Techniques
Northern Blott Analysis
J. Curtis (3/00)
Reagents
RNase protocol
Transfection Techniques
Amaxa protocol
Brain Slice Culture and Transfection Protocol
Lo Lab - Center for Drug Discovery.
Calcium Phosphate Transfection of Primary Neurons
Modified protocol by Jason Miller for significantly improved reproducibility, transfection efficiency, and survival. Contains significant differences from older Finkbeiner Lab protocol. Also contains recipes for all solutions needed. Posted January 2010.
Calcium Phosphate Transfection of PC12 Cells
M. Greenberg Lab
Six Well Cell Transfection of 293 Cells
Method devised by Marc Diamond
Transfection of NGF-differentiated PC12 Cells
Zhengui Xia (12/13/96)
Transfection of PC12 Cells with Lipofectamine
